Method for detecting magnesium chloride in edible mushrooms

[0022] Take a fresh white fungus sample without magnesium chloride, add 5% (weight percent) magnesium chloride, and crush it into a homogenate; weigh 20 g of the homogenate in a 250 mL Erlenmeyer flask, add 100 mL of water, shake and extract for 30 minutes, transfer to a 250 mL volumetric flask to constant volume to 250mL(V m ). The sample solution was centrifuged at 3000r / min for 10min, and the supernatant was collected for later use.

[0023] Pipette 50mL (V n ) above supernatant, placed in a 250mL Erlenmeyer flask, added 50mL water, 5mL triethanolamine solution (1+3), 10mL ammonia-ammonium chloride buffer solution (pH=10) and 5 drops of chrome black T indicator ( 5g / L), titrate with 0.002mol / L disodium edetate standard titration solution until the solution turns from purplish red to pure blue, and the volume of the final disodium edetate standard titration solution used is 4.53mL;

[0024] At the same time, water was used as a blank control, and the volume of the disodiu...

[0037] Take dried mushroom samples, add 20% (weight percentage content) of magnesium chloride, pulverize, and pass through a 40-mesh sieve for subsequent use. Weigh dry sample 2g, and all the other steps are the same as in Example 1.

[0038] where V 1 = 1.81 mL; V 0 =0.02mL; m=2g, and all the other variables are the same as in Example 1.

[0039] Finally, the weight percent content of magnesium chloride in the dry mushroom sample was calculated to be 18.2%, and the detection recovery rate was 91.0%.

[0041] With the method of embodiment one, do standard addition recovery test to blank sample fungus (fresh sample), the weight percent content of adding is respectively 1%, 5% and 20%, and detection recovery rate is all between 87.2%~92.3%, The specific data are shown in Table 1 below.

[0042] Table 1 Fungus (fresh sample) spike recovery experiment

[0043]